Our 3D cryoCLEM workflow is out!


Our team developed a creative solution for performing cryogenic correlative light and electron microscopy imaging in 3D samples, recently published in Communications Biology. We have developed a workflow, bridging 3D cryo light microscopy (LM) and 3D cryo FIB-SEM. By imprinting a recognizable and reproducible pattern on the top of high-pressure frozen samples using FinderTOP, we can find our way to 3D correlate LM and FIB-SEM. Read our paper and discover the possibilities of 3D cryoCLEM. 

de Beer, Marit, et al. "Precise targeting for 3D cryo-correlative light and electron microscopy volume imaging of tissues using a FinderTOP." Communications Biology 6.1 (2023): 510.